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KMID : 1134820190480101105
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Journal of the Korean Society of Food Science and Nutrition
2019 Volume.48 No. 10 p.1105 ~ p.1111
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Method Validation and Quantification of Lignans in the Fruits of Korean Omija (Schisandra chinensis) by Cultivation Region
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Hwang Byung-Soon
Kim Gi-Chang
Park Young-Hee
Hwang In-Guk
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Abstract
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This study examined the lignans (schizandrin, schizandrin A, gomisin A, gomisin G, gomisin H, gomisin J, and gomisin N) contents in the fruit of Omija (Schisandra chinensis) according to the cultivation region. The methods for determining lignans were validated by measuring the specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), precision, and accuracy using HPLC analysis. Among the lignans, schizandrin was the most abundant (3.85∼6.60 mg/g) followed by gomisin N (2.69∼4.38 mg/g). Schizandrin A and gomisin A were approximately 0.60∼2.04 and 0.86∼1.63 mg/g, respectively, and gomisin G, gomisin H, and gomisin J were less than 0.50 mg/g. The contents of seven lignans of Omija were 9.04∼14.90 mg/g. Omija showed different contents of lignans according to the cultivation region, but there was no significant difference in the total contents. These results will provide valuable basic data for the standardization of Korean Omija.
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KEYWORD
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Schisandra chinensis, lignan, HPLC
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